Pengenalpastian dan Profil Pengekspresan Gen Biosintesis Asid Amino Yis Psikrofil, Glaciozyma Antarctica
نویسندگان
چکیده
منابع مشابه
Crystal structure of fuculose aldolase from the Antarctic psychrophilic yeast Glaciozyma antarctica PI12.
Fuculose-1-phosphate aldolase (FucA) catalyses the reversible cleavage of L-fuculose 1-phosphate to dihydroxyacetone phosphate (DHAP) and L-lactaldehyde. This enzyme from mesophiles and thermophiles has been extensively studied; however, there is no report on this enzyme from a psychrophile. In this study, the gene encoding FucA from Glaciozyma antarctica PI12 (GaFucA) was cloned and the enzyme...
متن کاملMolecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12
BACKGROUND Cold-adapted enzymes are proteins produced by psychrophilic organisms that display a high catalytic efficiency at extremely low temperatures. Chitin consists of the insoluble homopolysaccharide β-(1, 4)-linked N-acetylglucosamine, which is the second most abundant biopolymer found in nature. Chitinases (EC 3.2.1.14) play an important role in chitin recycling in nature. Biodegradation...
متن کاملMolecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12
Psychrophilic basidiomycete yeast, Glaciozyma antarctica strain PI12, was shown to be a protease-producer. Isolation of the PI12 protease gene from genomic and mRNA sequences allowed determination of 19 exons and 18 introns. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp, coded for 963 amino ac...
متن کاملfile://C:\Documents and Settings\Dan Hogan\My Documents\FAF Gen
The author meta-analyzed studies comparing child adjustment in joint physical or joint legal custody with sole-custody settings, including comparisons with paternal custody and intact families where possible. Children in joint physical or legal custody were better adjusted than children in sole-custody settings, but no different from those in intact families. More positive adjustment of joint-c...
متن کاملYang Dan
Yang Dan Howard Hughes Medical Institute and the University of California, Berkeley Yang Dan is a Paul Licht Distinguished Professor in Biology and an Investigator of the Howard Hughes Medical Institute at the University of California, Berkeley. She grew up in Beijing and studied physics as an undergraduate student at Peking University. She conducted her PhD research in the lab of Mu-ming Poo a...
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ژورنال
عنوان ژورنال: Sains Malaysiana
سال: 2018
ISSN: 0126-6039
DOI: 10.17576/jsm-2018-4708-06